![]() ![]() For instance, diplococci refer to pairs of cocci, streptococci refer to chains and staphylococci refers to clusters of cocci.īacilli – These are rod-shaped bacteria and just like cocci they can be found on their own or grouped. They are named cocci due to their spherical shape, although they commonly appear in groups. There are a wide variety of different shapes, yet the three main types are cocci, bacilli, and spiral.Ĭocci – These are the most common type of bacteria. This mainly involves looking at their shape and size. Upon viewing the bacteria under the microscope, you will be able to identify the bacteria based on a wide variety of physical characteristics. Begin with a low magnification and gradually increase to observe greater detail. Place the glass slide on the microscope stage and begin viewing the specimen.Remove excess stain by running a gentle stream of water over the surface of the glass slide.Cover the slides in the chosen stain and leave for 1-2 minutes.Place your prepared slides next to each other on a paper towel or staining rack. ![]() Common stains used on bacteria include crystal violet, methylene blue, and safranin. Staining allows different structural components of the cells to be visualized including the cytoplasm, cell wall, and membranes. When looking at bacteria under the microscope much of the bacteria can appear transparent without staining. Mix the bacteria on the loop with the water you placed on the glass slide earlier and then flame your loop once again.Remember to maintain good aseptic technique to prevent contamination of the sample. Next, immediately remove a bacteria colony from the agar plate or broth using the loop.Allow the loop to glow red and then cool. Firstly, place the inoculation loop into the blue portion of the flame of the bunsen burner. The smear is now ready to be prepared.Using an inoculating loop, place a small drop of water whee you are going to place the bacteria and perform the smear.Using your felt marker, place a small mark where you will be placing the bacteria and making the smear.This involves placing a thin layer of bacteria onto a glass slide, typically before staining it. In order to do this, a bacterial smear must be performed. Once the bacteria has been left to incubate and grow on the agar plate, it then is viewed under the microscope. ![]() For example, blood agar is commonly used to identify bacteria that are responsible for hemolysis of the blood e.g.
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